Enhanced Aerobic Bio-remediation Report Introduction In this report, we present data collected on aerobicbio-remediation of soil contaminated with Perchloroethene (PCE), Trichloroethene (TCE), cis-1,2-Dichloroethene (cis12DCE) and Vinyl Chloride (VC) using VaporRemed. Enhanced Aerobic Bio-remediation of Chlorintaed Hydrocarbons In Situ Bioremediaton : When Does It Work?, lists at least three criteria for demonstrating that in situ bio-remediation is effective for a given site: Documented loss of contaminants from the site due to bio-remediation, Laboratory assays showing that the micro-organisms in site samples have the potential to transform contaminants under expected site condition, and One or more pieces of evidence showing that the bio-remediation potential is actually realized in the field.

VaporRemed Case Studies A site in Virginia A site in Virginia, one of our initial projects - circa 2006. An image showing the earmarked area An image showing the trenches below the AST A detailed report of the project Some quotes and figures from the report Groundwater results at MW-1 between 2005 - 2006 The application of AgroRemed appears to have reduced the levels of dissolved phase contamination in the groundwater and increased dispersion of the free product, resulting in an increase in the amount of free product in MW-4.

Mission statement - Evolving as we enhance our process to deal with various hydrocarbon contaminants Our mission is to help cleanup the mess created by fossil-fuels and dry-cleaning solvents such as Trichloroethylene (TCE), Perchloroethylene (PCE), asphalt and oil. Our mission is to cleanup of sites contaminated with dry cleaning chemicals such as PCE/TCE and also fuel oil releases in the environment effectively through aerobic bioremediation. Current cleanup methods are quite cumbersome and very expensive as they involve invasive process of excavation and disposal.

Plots of contamination levels at various locations Vapor Attenuation Curve between 2018 - 2020 IA-001 between 2018 - 2020 IA-002 between 2018 - 2020 IA-004 between 2018 - 2020 IA-007 between 2018 - 2020 IA-008 between 2018 - 2020 IA-009 between 2018 - 2020 IA-010 between 2018 - 2020 IA-011 between 2018 - 2019 SB-121 between Jun 2017 - Sept 2017 SG-106 between June 2019 - May 2020 SG-101 between June 2019 - May 2020 VP-003 between 2018 - 2020

Location details. Here are some images to help gain a perspective about the project. Mall’s front Geo probe Addition of AgroRemed

Update on the levels of PCE/TCE in Indoor Air (IA-001), SG-101 and SG-106 The current post presents the latest data recorded in indoor air in one of the hotspots, namely IA-001 in May 2020. IA-001 SG-101 SG-106 Note The increase in levels of 1,2-cisDCE and VinylChloride (VC) are indicative or bio-remediation of solvents accomplished by VaporRemed. TCE levels at IA-001 IA-001 SG-106 SG-101 Even though we see a slight uptick, the numbers are still within the site-specific limits.

First-order decay curves at SG-106 Here we focus on the project period between 12/4/2019 - 12/14/2019. The image shown below for the degradation of TCE/PCE levels after adding VaporRemed on 12/4/2019. These numbers allowed us to extrapolate a decay model for TCE based on the PCE data (an approximate second-order derivative). The image shown below shows an increase in TCE before falling off to a straight-line first-order decay. The curve shown above indicates that TCE levels increase before they decrease.

Comprehensive IAQ Report We post a summary of the comprehensive report for the site between 2018 and 2020. Report for 2020

Bacterial counts at VP-003. In order to determine whether Chlorine released during the bio-remediation of TCE/PCE is a limiting factor, we measured the bacterial counts at two points seperated by 70 hours. 3/23/2020 - 14, 800, 000 CFU/ml 3/25/2020 - 15, 300, 000 CFU/ml The sustained population count indicates that increase in levels of Chlorine due to bio-remediation of PCE and TCE are not impacting the bacterial population in VaporRemed.